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ผลงานตีพิมพ์ในวารสารวิชาการPotency of Indian gooseberry peel supplementation for suppressing rumen methane production via alteration of rumen microbiota: Batch culture evaluationsผู้แต่ง:Shinagawa, K., Mr.Phoompong BOONSAEN, Assistant Professor, Ms.Anchalee Khongpradit, Lecturer, Dr.Suriya Sawanon, Professor, Suzuki, Y., Koike, S., Kobayashi, Y., วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการHigh Loading Degradation of Poly(lactide)/Thermoplastic Starch Blend Film Using Mixed-Enzymes Produced by Fed-Batch Culture of Laceyella sacchari LP175ผู้แต่ง:Lomthong, T, Samaimai, S, Dr.Rangrong Yoksan, Associate Professor, Krajangsang, S, Dr.Vichien Kitpreechavanich, Associate Professor, วารสาร: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Factors Affecting Growth and ?-carotene Content of Chlorosarcinopsis sp. (PSU/CHL20) in Batch Culture) ผู้เขียน:Tassnapa Wongsnansilp, Pimpan Tansakul, Manop Arunyanart สื่อสิ่งพิมพ์:pdf AbstractThe effect of light intensity, potassium nitrate concentrations and temperature on the growth
and ?-carotene content of Chlorosarcinopsis sp. (PSU/CHL20) were determined. In the first experiment,
algal cells were grown on a shaker in Erlenmeyer flasks containing 150 ml of NSIII medium and at a
shaking speed of 150 rpm. Potassium nitrate concentrations were 0, 0.1 and 0.2 g l-1. Light intensities
used were 60, 120 and 180 ?molm-2s-1. The light / dark cycle was 16:8 h and the temperature was
controlled at 25?C. The optimum light intensity and potassium nitrate concentration for algal growth
and ?-carotene content was 120 ?molm-2s-1 and 0.1 g l-1 which produced a maximum cell number of
138?105 cell ml -1and a ?-carotene content of 0.133 mg l-1, (0.012 pg cell-1) respectively. In the second
experiment, the effect of temperature on the growth and ?-carotene content was investigated. In this
experiment the light intensity was 120 mmolm-2s-1 and the potassium nitrate concentration was 0.1g
l-1, at temperatures of 25?C, 30?C and 35?C. Although statistically insignificant (P>0.05), the algal
growth increased slightly with increasing temperature. The highest cell numbers of 148x105 cell ml-1
were obtained at 30?C on day 14 of cultivation. The maximum ?-carotene content of 0.543 mg l-1 (0.052
pg cell-1) was achieved at 30?C and this was significantly higher than that of the culture grown at 25?C
(P<0.05). |
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ที่มา:วารสารวิทยาศาสตร์และเทคโนโลยีหัวเรื่อง:อิทธิพลของสารสกัดจากสมุนไพรเอื้องหมายนาต่อการหมักในกระเพาะรูเมนในแบชคัลเจอร์ |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production and Partial Characterization of Chitosanases from a Newly Isolated Bacillus cereus) ผู้เขียน:Sutee Wangtueai, ดร.วันชัย วรวัฒนเมธีกุล, รองศาสตราจารย์, นางมัทนา แสงจินดาวงษ์, รองศาสตราจารย์, นางสาวนวลพรรณ ณ ระนอง, ดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe production of chitosanases by a newly isolated Bacillus cereus TP12.24 was studied both in shake flask and fermenter cultures. The M9-chitosan medium was found most suitable with 0.5% chitosan as a sole carbon source optimized under aerobic growth conditions at pH 6.0 and 30?C. The specific rates of growth, substrate consumption, and enzyme production were improved using controlled completely aerobic conditions in 2-l fermenter. While the yield of biomass was considerably increased, the enzyme yield was on the contrary decreased. As a result, the volumetric chitosanases productivity was 43.55 U/l h, which was 1.2 times that obtained from shake flask culture due to higher specific rates of chitosan consumption and chitosanases production. In this work, the crude chitosanases from Bacillus cereus TP12.24 showed their optimal pH and temperature at 6.5 and 55?C, while the stabilities to pH and temperature were found at 3.0-8.0 and 30-50?C, respectively. The Bacillus cereus chitosanases could be used for preparing the chitosano-oligosaccharides under mild temperature. |
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